Stands for "Graphical User Interface," and is pronounced "gooey." It refers to the graphical interface of a computer that allows users to click and drag objects with a mouse instead of entering text at a command line. Two of the most popular operating systems, Windows and the Mac OS, are GUI-based. The graphical user interface was first introduced to the public by Apple with the Macintosh in 1984. However, the idea was actually taken from an earlier user interface developed by Xerox.

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Definition from the PC Glossary